Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.
Identifieur interne : 000230 ( Main/Exploration ); précédent : 000229; suivant : 000231Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.
Auteurs : Lorena Noelia Sendín [Argentine] ; Ingrid Georgina Orce [Argentine] ; Rocío Liliana G Mez [Argentine] ; Ram N Enrique [Argentine] ; Carlos Froilán Grellet Bournonville [Argentine] ; Aldo Sergio Noguera [Argentine] ; Adrián Alberto Vojnov [Argentine] ; María Rosa Marano [Argentine] ; Atilio Pedro Castagnaro [Argentine] ; María Paula Filippone [Argentine]Source :
- Plant molecular biology [ 1573-5028 ] ; 2017.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Capsicum (génétique), Citrus sinensis (génétique), Citrus sinensis (microbiologie), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Pousses de plante (génétique), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Résistance à la maladie (génétique), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH), Xanthomonas campestris (pathogénicité).
- MESH :
- génétique : Agrobacterium tumefaciens, Capsicum, Citrus sinensis, Maladies des plantes, Pousses de plante, Résistance à la maladie.
- microbiologie : Citrus sinensis, Maladies des plantes.
- pathogénicité : Xanthomonas campestris.
- Régions promotrices (génétique), Régulation de l'expression des gènes végétaux, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Capsicum (genetics), Citrus sinensis (genetics), Citrus sinensis (microbiology), Disease Resistance (genetics), Gene Expression Regulation, Plant (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Shoots (genetics), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (MeSH), Transformation, Genetic (MeSH), Xanthomonas campestris (pathogenicity).
- MESH :
- genetics : Agrobacterium tumefaciens, Capsicum, Citrus sinensis, Disease Resistance, Plant Diseases, Plant Shoots.
- microbiology : Citrus sinensis, Plant Diseases.
- pathogenicity : Xanthomonas campestris.
- Gene Expression Regulation, Plant, Plants, Genetically Modified, Promoter Regions, Genetic, Transformation, Genetic.
Abstract
Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduced canker formation and induced plant defence mechanisms. In this work, the effect of the stable expression of Bs2 gene on citrus canker resistance was evaluated in transgenic plants of Citrus sinensis cv. Pineapple. Interestingly, Agrobacterium-mediated transformation of epicotyls was unsuccessful when a constitutive promoter (2× CaMV 35S) was used in the plasmid construction, but seven transgenic lines were obtained with a genetic construction harbouring Bs2 under the control of a pathogen-inducible promoter, from glutathione S-transferase gene from potato. A reduction of disease symptoms of up to 70% was observed in transgenic lines expressing Bs2 with respect to non-transformed control plants. This reduction was directly dependent on the Xcc avrBs2 gene since no effect was observed when a mutant strain of Xcc with a disruption in avrBs2 gene was used for inoculations. Additionally, a canker symptom reduction was correlated with levels of the Bs2 expression in transgenic plants, as assessed by real-time qPCR, and accompanied by the production of reactive oxygen species. These results indicate that the pepper Bs2 resistance gene is also functional in a family other than the Solanaceae, and could be considered for canker control.
DOI: 10.1007/s11103-017-0586-8
PubMed: 28155188
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.</title><author><name sortKey="Sendin, Lorena Noelia" sort="Sendin, Lorena Noelia" uniqKey="Sendin L" first="Lorena Noelia" last="Sendín">Lorena Noelia Sendín</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Orce, Ingrid Georgina" sort="Orce, Ingrid Georgina" uniqKey="Orce I" first="Ingrid Georgina" last="Orce">Ingrid Georgina Orce</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="G Mez, Rocio Liliana" sort="G Mez, Rocio Liliana" uniqKey="G Mez R" first="Rocío Liliana" last="G Mez">Rocío Liliana G Mez</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Enrique, Ram N" sort="Enrique, Ram N" uniqKey="Enrique R" first="Ram N" last="Enrique">Ram N Enrique</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Grellet Bournonville, Carlos Froilan" sort="Grellet Bournonville, Carlos Froilan" uniqKey="Grellet Bournonville C" first="Carlos Froilán" last="Grellet Bournonville">Carlos Froilán Grellet Bournonville</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Noguera, Aldo Sergio" sort="Noguera, Aldo Sergio" uniqKey="Noguera A" first="Aldo Sergio" last="Noguera">Aldo Sergio Noguera</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Vojnov, Adrian Alberto" sort="Vojnov, Adrian Alberto" uniqKey="Vojnov A" first="Adrián Alberto" last="Vojnov">Adrián Alberto Vojnov</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468, C1440FFX, Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468, C1440FFX, Buenos Aires</wicri:regionArea><wicri:noRegion>Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Marano, Maria Rosa" sort="Marano, Maria Rosa" uniqKey="Marano M" first="María Rosa" last="Marano">María Rosa Marano</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Bioquímica y Farmacia-Instituto de Biología Celular y Molecular de Rosario, Universidad Nacional de Rosario, Suipacha 590, S2002LRK, Rosario, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Facultad de Bioquímica y Farmacia-Instituto de Biología Celular y Molecular de Rosario, Universidad Nacional de Rosario, Suipacha 590, S2002LRK, Rosario</wicri:regionArea><wicri:noRegion>Rosario</wicri:noRegion></affiliation></author><author><name sortKey="Castagnaro, Atilio Pedro" sort="Castagnaro, Atilio Pedro" uniqKey="Castagnaro A" first="Atilio Pedro" last="Castagnaro">Atilio Pedro Castagnaro</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Filippone, Maria Paula" sort="Filippone, Maria Paula" uniqKey="Filippone M" first="María Paula" last="Filippone">María Paula Filippone</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina. paulafilippone@eeaoc.org.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28155188</idno><idno type="pmid">28155188</idno><idno type="doi">10.1007/s11103-017-0586-8</idno><idno type="wicri:Area/Main/Corpus">000249</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000249</idno><idno type="wicri:Area/Main/Curation">000249</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000249</idno><idno type="wicri:Area/Main/Exploration">000249</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.</title><author><name sortKey="Sendin, Lorena Noelia" sort="Sendin, Lorena Noelia" uniqKey="Sendin L" first="Lorena Noelia" last="Sendín">Lorena Noelia Sendín</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Orce, Ingrid Georgina" sort="Orce, Ingrid Georgina" uniqKey="Orce I" first="Ingrid Georgina" last="Orce">Ingrid Georgina Orce</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="G Mez, Rocio Liliana" sort="G Mez, Rocio Liliana" uniqKey="G Mez R" first="Rocío Liliana" last="G Mez">Rocío Liliana G Mez</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Enrique, Ram N" sort="Enrique, Ram N" uniqKey="Enrique R" first="Ram N" last="Enrique">Ram N Enrique</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Grellet Bournonville, Carlos Froilan" sort="Grellet Bournonville, Carlos Froilan" uniqKey="Grellet Bournonville C" first="Carlos Froilán" last="Grellet Bournonville">Carlos Froilán Grellet Bournonville</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Noguera, Aldo Sergio" sort="Noguera, Aldo Sergio" uniqKey="Noguera A" first="Aldo Sergio" last="Noguera">Aldo Sergio Noguera</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Vojnov, Adrian Alberto" sort="Vojnov, Adrian Alberto" uniqKey="Vojnov A" first="Adrián Alberto" last="Vojnov">Adrián Alberto Vojnov</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468, C1440FFX, Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468, C1440FFX, Buenos Aires</wicri:regionArea><wicri:noRegion>Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Marano, Maria Rosa" sort="Marano, Maria Rosa" uniqKey="Marano M" first="María Rosa" last="Marano">María Rosa Marano</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Bioquímica y Farmacia-Instituto de Biología Celular y Molecular de Rosario, Universidad Nacional de Rosario, Suipacha 590, S2002LRK, Rosario, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Facultad de Bioquímica y Farmacia-Instituto de Biología Celular y Molecular de Rosario, Universidad Nacional de Rosario, Suipacha 590, S2002LRK, Rosario</wicri:regionArea><wicri:noRegion>Rosario</wicri:noRegion></affiliation></author><author><name sortKey="Castagnaro, Atilio Pedro" sort="Castagnaro, Atilio Pedro" uniqKey="Castagnaro A" first="Atilio Pedro" last="Castagnaro">Atilio Pedro Castagnaro</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Filippone, Maria Paula" sort="Filippone, Maria Paula" uniqKey="Filippone M" first="María Paula" last="Filippone">María Paula Filippone</name><affiliation wicri:level="1"><nlm:affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina. paulafilippone@eeaoc.org.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant molecular biology</title><idno type="eISSN">1573-5028</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Capsicum (genetics)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (microbiology)</term><term>Disease Resistance (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Shoots (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Xanthomonas campestris (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Capsicum (génétique)</term><term>Citrus sinensis (génétique)</term><term>Citrus sinensis (microbiologie)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Pousses de plante (génétique)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Résistance à la maladie (génétique)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Xanthomonas campestris (pathogénicité)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Capsicum</term><term>Citrus sinensis</term><term>Disease Resistance</term><term>Plant Diseases</term><term>Plant Shoots</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Capsicum</term><term>Citrus sinensis</term><term>Maladies des plantes</term><term>Pousses de plante</term><term>Résistance à la maladie</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Citrus sinensis</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Xanthomonas campestris</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Xanthomonas campestris</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduced canker formation and induced plant defence mechanisms. In this work, the effect of the stable expression of Bs2 gene on citrus canker resistance was evaluated in transgenic plants of Citrus sinensis cv. Pineapple. Interestingly, Agrobacterium-mediated transformation of epicotyls was unsuccessful when a constitutive promoter (2× CaMV 35S) was used in the plasmid construction, but seven transgenic lines were obtained with a genetic construction harbouring Bs2 under the control of a pathogen-inducible promoter, from glutathione S-transferase gene from potato. A reduction of disease symptoms of up to 70% was observed in transgenic lines expressing Bs2 with respect to non-transformed control plants. This reduction was directly dependent on the Xcc avrBs2 gene since no effect was observed when a mutant strain of Xcc with a disruption in avrBs2 gene was used for inoculations. Additionally, a canker symptom reduction was correlated with levels of the Bs2 expression in transgenic plants, as assessed by real-time qPCR, and accompanied by the production of reactive oxygen species. These results indicate that the pepper Bs2 resistance gene is also functional in a family other than the Solanaceae, and could be considered for canker control.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28155188</PMID><DateCompleted><Year>2017</Year><Month>04</Month><Day>03</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-5028</ISSN><JournalIssue CitedMedium="Internet"><Volume>93</Volume><Issue>6</Issue><PubDate><Year>2017</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant molecular biology</Title><ISOAbbreviation>Plant Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.</ArticleTitle><Pagination><MedlinePgn>607-621</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11103-017-0586-8</ELocationID><Abstract><AbstractText>Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduced canker formation and induced plant defence mechanisms. In this work, the effect of the stable expression of Bs2 gene on citrus canker resistance was evaluated in transgenic plants of Citrus sinensis cv. Pineapple. Interestingly, Agrobacterium-mediated transformation of epicotyls was unsuccessful when a constitutive promoter (2× CaMV 35S) was used in the plasmid construction, but seven transgenic lines were obtained with a genetic construction harbouring Bs2 under the control of a pathogen-inducible promoter, from glutathione S-transferase gene from potato. A reduction of disease symptoms of up to 70% was observed in transgenic lines expressing Bs2 with respect to non-transformed control plants. This reduction was directly dependent on the Xcc avrBs2 gene since no effect was observed when a mutant strain of Xcc with a disruption in avrBs2 gene was used for inoculations. Additionally, a canker symptom reduction was correlated with levels of the Bs2 expression in transgenic plants, as assessed by real-time qPCR, and accompanied by the production of reactive oxygen species. These results indicate that the pepper Bs2 resistance gene is also functional in a family other than the Solanaceae, and could be considered for canker control.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sendín</LastName><ForeName>Lorena Noelia</ForeName><Initials>LN</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Orce</LastName><ForeName>Ingrid Georgina</ForeName><Initials>IG</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gómez</LastName><ForeName>Rocío Liliana</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Enrique</LastName><ForeName>Ramón</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grellet Bournonville</LastName><ForeName>Carlos Froilán</ForeName><Initials>CF</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noguera</LastName><ForeName>Aldo Sergio</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vojnov</LastName><ForeName>Adrián Alberto</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468, C1440FFX, Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marano</LastName><ForeName>María Rosa</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Facultad de Bioquímica y Farmacia-Instituto de Biología Celular y Molecular de Rosario, Universidad Nacional de Rosario, Suipacha 590, S2002LRK, Rosario, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Castagnaro</LastName><ForeName>Atilio Pedro</ForeName><Initials>AP</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Filippone</LastName><ForeName>María Paula</ForeName><Initials>MP</Initials><AffiliationInfo><Affiliation>Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina. paulafilippone@eeaoc.org.ar.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>02</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Plant Mol Biol</MedlineTA><NlmUniqueID>9106343</NlmUniqueID><ISSNLinking>0167-4412</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002212" MajorTopicYN="N">Capsicum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016959" MajorTopicYN="N">Xanthomonas campestris</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium-mediated transformation</Keyword><Keyword MajorTopicYN="N">AvrBs2 gene</Keyword><Keyword MajorTopicYN="N">Citrus breeding</Keyword><Keyword MajorTopicYN="N">Glutathione S-transferase promoter</Keyword><Keyword MajorTopicYN="N">Resistance genes</Keyword><Keyword MajorTopicYN="N">Xanthomonas citri subsp. citri</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>07</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>01</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>2</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>4</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>2</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28155188</ArticleId><ArticleId IdType="doi">10.1007/s11103-017-0586-8</ArticleId><ArticleId IdType="pii">10.1007/s11103-017-0586-8</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7819-24</Citation><ArticleIdList><ArticleId IdType="pubmed">9636234</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechniques. 1991 Oct;11(4):428-32</Citation><ArticleIdList><ArticleId IdType="pubmed">1793572</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1993 Aug;5(8):921-930</Citation><ArticleIdList><ArticleId IdType="pubmed">12271090</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2003 Mar;216(5):802-14</Citation><ArticleIdList><ArticleId IdType="pubmed">12624768</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1996 Oct;8(10):1793-1807</Citation><ArticleIdList><ArticleId IdType="pubmed">12239362</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14153-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10570214</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1990 Jul 26;346(6282):385-6</Citation><ArticleIdList><ArticleId IdType="pubmed">2374611</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2003 May 1;4(3):141-57</Citation><ArticleIdList><ArticleId IdType="pubmed">20569374</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2006 Feb;15(1):83-93</Citation><ArticleIdList><ArticleId IdType="pubmed">16475012</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1998 Aug;10(8):1251-66</Citation><ArticleIdList><ArticleId IdType="pubmed">9707527</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Microbiol. 1998 Feb;6(2):54-61</Citation><ArticleIdList><ArticleId IdType="pubmed">9507639</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2008 Aug;6(6):585-608</Citation><ArticleIdList><ArticleId IdType="pubmed">18444970</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:251-275</Citation><ArticleIdList><ArticleId IdType="pubmed">15012264</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1995 Oct;7(10):1529-1536</Citation><ArticleIdList><ArticleId IdType="pubmed">12242354</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8776-81</Citation><ArticleIdList><ArticleId IdType="pubmed">8710948</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2004 Jan 1;5(1):1-15</Citation><ArticleIdList><ArticleId IdType="pubmed">20565577</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2008 Oct;17 (5):873-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18306055</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1995 Jul;14(10):616-9</Citation><ArticleIdList><ArticleId IdType="pubmed">24194307</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2006 Feb;15(1):57-68</Citation><ArticleIdList><ArticleId IdType="pubmed">16475010</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 1996 Dec;9(9):850-5</Citation><ArticleIdList><ArticleId IdType="pubmed">8969533</ArticleId></ArticleIdList></Reference><Reference><Citation>ScientificWorldJournal. 2013 Jul 25;2013:491207</Citation><ArticleIdList><ArticleId IdType="pubmed">23983635</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biol (Stuttg). 2008 Jul;10 (4):451-61</Citation><ArticleIdList><ArticleId IdType="pubmed">18557905</ArticleId></ArticleIdList></Reference><Reference><Citation>Syst Appl Microbiol. 2005 Aug;28(6):494-518</Citation><ArticleIdList><ArticleId IdType="pubmed">16104350</ArticleId></ArticleIdList></Reference><Reference><Citation>J Microbiol Methods. 2006 Jul;66(1):104-15</Citation><ArticleIdList><ArticleId IdType="pubmed">16414133</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2007 Jan;26(1):39-45</Citation><ArticleIdList><ArticleId IdType="pubmed">16927091</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1996 Aug;178(15):4661-9</Citation><ArticleIdList><ArticleId IdType="pubmed">8755898</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1993 Jan;236(2-3):179-86</Citation><ArticleIdList><ArticleId IdType="pubmed">8437562</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><country name="Argentine"><noRegion><name sortKey="Sendin, Lorena Noelia" sort="Sendin, Lorena Noelia" uniqKey="Sendin L" first="Lorena Noelia" last="Sendín">Lorena Noelia Sendín</name></noRegion><name sortKey="Castagnaro, Atilio Pedro" sort="Castagnaro, Atilio Pedro" uniqKey="Castagnaro A" first="Atilio Pedro" last="Castagnaro">Atilio Pedro Castagnaro</name><name sortKey="Enrique, Ram N" sort="Enrique, Ram N" uniqKey="Enrique R" first="Ram N" last="Enrique">Ram N Enrique</name><name sortKey="Filippone, Maria Paula" sort="Filippone, Maria Paula" uniqKey="Filippone M" first="María Paula" last="Filippone">María Paula Filippone</name><name sortKey="G Mez, Rocio Liliana" sort="G Mez, Rocio Liliana" uniqKey="G Mez R" first="Rocío Liliana" last="G Mez">Rocío Liliana G Mez</name><name sortKey="Grellet Bournonville, Carlos Froilan" sort="Grellet Bournonville, Carlos Froilan" uniqKey="Grellet Bournonville C" first="Carlos Froilán" last="Grellet Bournonville">Carlos Froilán Grellet Bournonville</name><name sortKey="Marano, Maria Rosa" sort="Marano, Maria Rosa" uniqKey="Marano M" first="María Rosa" last="Marano">María Rosa Marano</name><name sortKey="Noguera, Aldo Sergio" sort="Noguera, Aldo Sergio" uniqKey="Noguera A" first="Aldo Sergio" last="Noguera">Aldo Sergio Noguera</name><name sortKey="Orce, Ingrid Georgina" sort="Orce, Ingrid Georgina" uniqKey="Orce I" first="Ingrid Georgina" last="Orce">Ingrid Georgina Orce</name><name sortKey="Vojnov, Adrian Alberto" sort="Vojnov, Adrian Alberto" uniqKey="Vojnov A" first="Adrián Alberto" last="Vojnov">Adrián Alberto Vojnov</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000230 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000230 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28155188
|texte= Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28155188" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |